See Also: Rothera's nitroprusside test(medicine)
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Rothera's nitroprusside test (medicine)


Rothera's nitroprusside test


A test for ketone bodies; 5 ml of fresh urine are saturated with solid ammonium sulfate and mixed with 10 drops of freshly prepared 2% sodium nitroprusside solution, which is then mixed with 10 drops of concentrated ammonia water and allowed to stand for 15 min; the presence of acetoacetic acid, or of larger concentrations of acetone, is indicated by the development of a blue-purple colour.