See Also: dideoxy sequencing(medicine)
dideoxy procedure(medicine)
Herbal medicine (botanical medicine, herbology, phytomedicine)(health)
sequencing(dictionary)
sequencing(encyclopedia)
Sequencing(medicine)
DNA sequencing(medicine)
gene sequencing(medicine)
protein sequencing(medicine)
Directed DNA sequencing(health)

dideoxy sequencing (medicine)


dideoxy sequencing


The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands.