See Also: media vuelta(dictionary)
vuelta(dictionary)
dideoxy procedure(medicine)
dideoxy sequencing(medicine)
Herbal medicine (botanical medicine, herbology, phytomedicine)(health)
Medicine Lodge Memorial Hospital- Medicine Lodge(health)
Orthomolecular medicine (orthomolecular nutritional medicine, orthomolecular therapy)(health)
Dex Media(finance)
new media(dictionary)
Media(medicine)

dideoxy sequencing (medicine) and media vuelta (iou)


dideoxy sequencing (medicine)


dideoxy sequencing


The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands.


media vuelta (iou)



media vuelta noun phr. ['medja 'vwelta] M20.
[Spanish, lit. 'half-turn'.]
Bullfighting. A method of killing a bull by approaching and stabbing it from behind.